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DiAtomic O2xygen Info

Antimicrobial, Chemical Assay and Oxygen Test Reports Summary (January, 1996):

1. Nelson Laboratories, Inc. of Salt Lake City, UT completed its Antimicrobial Preservative Effect Test (Lab Number 72984B) for Di-Atomic Oxygen, the Stabilized Oxygen Supplement ( Batch SL6) at 100% concentration on March 13, 1995 against the following organisms:

Bacteria: Staphylococcus Aureus (ATCC 6538); Pseudomonas Aeruginosa (ATCC 9027); Escherichia Coli (ATCC 8739)
Yeast: Candida Albicans (ATCC 10231)
Mould: Aspergillus Niger (ATCC 16404)

Organisms were assayed for surviving organisms after 0 hours (immediately after application of Di-Atomic Oxygen and at 7, 14, 21 and 28 days.   According to U.S. Pharmacopeia, a preservative is considered effective if the number of bacteria recovered is not more than 0.1% of the initial concentration by the 14th day; the number of moulds and yeasts recovered per ml remains at or below the initial concentration during the first 14 days; the number of organisms per ml remains at or below the designated levels during the remainder of the 28 day period.  Nelson Labs reported:

"The test product reduced all of the challenge organisms below the detectable limit at time 0 sample interval.  No organisms were recovered from the product throughout the remainder of the test.  The test product (Di-Atomic Oxygen) meets the USP requirements for an effective antimicrobial preservative against the standard test organisms."

2. Nelson Laboratories, Inc. of Salt Lake City completed its Modified Antimicrobial Preservative Effect Test (Lab Number 75705) for Di-Atomic Oxygen, the Stabilized Oxygen Supplement (Batch SL6) at 50% concentration on April 10, 1995 (Laboratory Number 75705), protocol Number 950833-1) against the following organisms:

Bacteria: Staphylococcus Aureus (ATCC 6538); Pseudomonas Aeruginosa (ATCC 9027); Escherichia Coli (ATCC 8739)
Yeast: Candida Albicans (ATCC 10231)
Mould: Aspergillus Niger (ATCC 16404); Aspergillus Flavus (ATCC 9643)

Organisms were assayed for surviving organisms after 0 hours (immediately after application of Di-Atomic Oxygen) and at 24 hours.  The table below is Nelson Lab's summary which indicates that all four organisms were effectively controlled at both 0 hour and at 24 hours 99.9%.

Test Organism

Sample Test time Elapsed
Control 0 Hours 24 Hours
Staphylococcus Aureus
(ATCC 6538)
CFU/ml
count

Percent Reduction
1,193,333
 

N/A

< 10


99.9%

< 10


99.9%
Pseudomonas Aeruginosa
(ATCC 9027)
CFU/ml
count

Percent Reduction
3,873,333


N/A
< 10


99.9%
< 10


99.9%
Esheriria Coli
(ATCC 8739)
CFU/ml
count

Percent Reduction
1,303,333


N/A
< 10


99.9%
< 10


99.9%
Candida Albicans
(ATCC 10231)
CFU/ml
count

Percent Reduction
1,180,000


N/A
< 10


99.9%
< 10


99.9%
Aspergillus Niger
(ATCC 16404)
CFU/ml
count

Percent Reduction
297,333


N/A
< 10


99.5%
< 10


99.9%
Aspergillus Flavus
(ATCC 9643)
CFU/ml
count

Percent Reduction
1,742,333


N/A
< 10


99.5%
< 10


99.9%

3. Dr. Joseph Montelcalvo, Jr., PhD, of Central Coast Consulting, completed a preliminary study of the antimicrobial effect of a 1% solution of Di-Atomic Oxygen on Cat's Claw (herb) on July 25th, 1995.  He wrote: "We find that Di-Atomic Oxygen is very effective when used as a sanitizing disinfectant on the Cat's Claw.  The results of this study show that on average the 1% SL-12-1 product showed a reduction of 85.7% of total moulds on the powder and 82.9% reduction on the chips...Based on these findings, Di-Atomic Oxygen shows great promise as a sanitizing agent for herbs, spices and other agricultural products where a significant reduction in mould colony  units is required to enhance / maintain  quality, reduce spoilage and extend shelf life."

4. Dr. Joseph Montelcalvo, Jr., PhD, of Central Coast Consulting, completed a second study of the antimicrobial effect of a 3.5% solution of Di-Atomic Oxygen on Cat's Claw (herb) chips on October 12, 1995.  He concluded: "Di-Atomic Oxygen showed a percent mould reduction of 85.56% and 94.23% after 5 and 15 minutes respectively.  Yeast reduction as a function of Di-Atomic Oxygen treatment was 88.19% and 91.1% after 5 and 15 minutes of treatment.  These results clearly indicate that Di-Atomic Oxygen treatment at 3.5% level can reduce over 94% of the level of mould and over 91% of yeast cell contamination...On the basis of the data obtained from this study, Di-Atomic Oxygen is very effective and its effectiveness should be relatively the same on agricultural products such as apples, pears, grapes, carrots, etc...Therefore, there is great need in the industry to find a sanitizing compound that could take the place of chlorine (Chlorine Dioxide). Di-Atomic Oxygen shows promise based on the available data and information provided to me to date."

Chemical Assay Tests:

1. Chemtech Analytical Laboratory in Salt Lake City, UT completed a certificate of analysis (Lab #U011783) on ten drops of Di-Atomic Oxygen (Batch SL18B) in 8 ounces of distilled water on July 27th, 1994.  The lab's analysis determined that the TDS level in mg/L was 202 with almost every parameter test near or below the lab's minimum detection levels.  Only Chloride (at 145 ppm or mg/L).   Nothing on the lab analysis approaches FDA's or EPA's maximum contaminate levels nor is an unapproved constituent in a food supplement.

2. Pace Incorporated Environmental Laboratories in Camarillo, CA completed a laboratory analysis (Lab # CK-5540-1) on a 100% solution of Di-Atomic Oxygen on November 15, 1994.  The lab determined that Di-Atomic Oxygen contains no sodium chlorite (NaClO2) nor any chlorite (ClO2).

3.Pace Incorporated Environmental Laboratories in Camarillo, CA completed a laboratory analysis (Lab # CK-6317-4) on a 100% solution of Di-Atomic Oxygen on January 4th, 1994.  It was determined that Di-Atomic Oxygen contains trace nutritional minerals including Calcium (52 mg/L) and magnesium (23 mg/L).

4. John H. MacDonald, Ph.D, Senior Scientist at USANA, Inc. in Salt Lake City, UT, analysed Chemtech Di-Atomic Oxygen Assay Report #U011783-SL-18_B sampled 7/21/94 and determined: "I can find no substance with levels high enough to indicate toxicity to anyone consuming Di-Atomic Oxygen. the sodium levels at only 9.24 ppm are well below the recommended daily reference values determined by the U.S. Government.  Toxic metals (lead, mercury & arsenic) are virtually undetectable as are nitrate levels and other trace minerals.  Di-Atomic Oxygen, in its current formulation, is totally safe to the human body.

FDA Recognition:

M. Val Miller, Attorney at Law, specialist in FDA regulations and former Associate Chief Counsel for the FDA in Washington, D.C. wrote: "Clearly, Di-Atomic Oxygen   product is subject to the conditions of the D.S.H.E.A. (Dietray and Supplement Health Education Act).  By definition, a dietary supplement means, 'a product intended to supplement the diet by increasing the total dietary intake that bears or contains one or more of the following dietary ingredients: a vitamin, a mineral, an herb or other botanical...' (See 21 USC p. 321 et. seq.) Di-Atomic Oxygen the stabilized oxygen supplement is, as a matter of law, a dietary supplement."

Thomas L. Sawyer, Director, Compliance Branch, Department of Health and Human Services, Public Health Service Food and Drug Administration, wrote on October 5, 1995: "We have reviewed your revised package label, container label and promotional material submitted and have the following comments.   The product Di-Atomic Oxygen is a dietary supplement as defined in Section 201(f) of the Federal Food, Drug, and Cosmetic Act (the Act)."

The following information is provide for reference purposes. It does not represent that this information infers or represents that its product Di-Atomic Oxygen is designed or may be used for any medical purpose nor is the provided information intended to diagnose or treat any medical condition.  Di-Atomic Oxygen the oxygen supplement is designed and sold as a dietary supplement under the strict regulations and guidelines of the United States Federal Dietary and Health Education Act of 1995. 

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